Our research focuses on exploiting microfabrication to miniaturize chemical and biochemical analysis technologies. Using microfluidic of channels on glass devices, we have created networks for primarily electrophoresis-based separations for genotyping and sequencing of DNA. These microfluidic concepts have also been applied to making PCR reactors directly coupled to CE channels, and the micro-plumbing necessary for them. Expanding on these ideas, we're also developing an amino-acid analyzer for extraterrestrial exploration, and complex networks for DNA-based computing. Building upon the possibilities of microfabrication, we're also exploring integrated detectors - both electrochemical and optical.
DNA Sequencers
The 96-lane microfabricated sequencer we developed pushes the envelope of high-speed sequencing, yielding an impressive 1700 bases/min of PHRED 20 or better quality. The design incomporates 16-cm folded channels, and fluidically balanced injectors derived from both simulation and experiment.
DNA Genotypers
With our revolutionary rotary confocal fluorescence scanner, we've been able to develop arrays of 12 to 384 microfabricated capillaries on glass wafers from 4 to 8" in diameter making possible high-throughput genotyping of > 1 sample per second.
Pathogen Detection
We have built an integrated cell capture-PCR-capillary electrophoresis microdevice as part of a portable pathogen detection system. This lab-on-a-chip system incorporates microfabricated heaters and temperture sensors for PCR thernal cycling , PDMS membrane valves for microfluidic manipulation, and capture chambers for sample purification of pathogenic cells.
